Recombinant Human Glucokinase/GCK Protein Summary
| Description |
A recombinant protein corresponding to 470 amino acids of Human pancreas Glucokinase/GCK
Source: E. coli. Amino Acid Sequence: The amino acid sequence of this product is not available. |
| Preparation Method |
E. coli
|
| Details of Functionality |
303 pmol/min/ug. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30C for 30 min.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
GCK
|
Applications/Dilutions
| Theoretical MW |
53.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
|
| Concentration |
1 mg/ml
|
Alternate Names for Recombinant Human Glucokinase/GCK Protein
- EC 2.7.1
- EC 2.7.1.2
- FGQTL3
- GCK
- GK
- GLK
- glucokinase (hexokinase 4)
- Glucokinase
- Hexokinase-4
- Hexokinase-D
- HHF3
- HK4
- HK4pancreatic isozyme
- HKIV
- HXKP
- LGLK
- maturity onset diabetes of the young 2
- MODY2
Background
Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.