RelA/NFkB p65 Antibody (2A12A7) Summary
| Immunogen |
Synthetic peptide from human NFkB.
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Rabbit
|
| Gene |
RELA
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western blot analysis (1-4 ug/ml). However, the optimal conditions should be determined individually. A431 cells can be used as a positive control.
|
| Theoretical MW |
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS, 1.0 mg/ml BSA and 50% Glycerol. Preservative: 1.5mM Sodium Azide
|
| Preservative |
Sodium Azide
|
| Concentration |
0.2 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for RelA/NFkB p65 Antibody (2A12A7)
- MGC131774
- nf kb p65
- NFkB p65
- NF-kB p65
- NFKB3
- NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
- Nuclear factor NF-kappa-B p65 subunit
- Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
- p65
- p65RelA
- rela p65
- RelA
- v-rel reticuloendotheliosis viral oncogene homolog A (avian)
- v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65
Background
NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that “supershift” the DNA/protein complex.