Lck [p Tyr505] Antibody Summary
| Immunogen |
Synthetic peptide corresponding to amino acids around Tyr505 of human Lck.
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| Modification |
p Tyr505
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
LCK
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Western blotting (1-2 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (20-25 ug/ml). However, the optimal conditions should be determined individually. The antibody detects 56 kDa phosphorylated Lck (Tyr505) of human, mouse and rat origins. Reactivity to other species has not been tested.
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| Reviewed Applications |
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Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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| Buffer |
PBS (pH 7.2), 1.0% BSA and 50% Glycerol
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for Lck [p Tyr505] Antibody
- EC 2.7.10
- EC 2.7.10.2
- Hck-3
- Lck
- Lcktkr
- Leukocyte C-terminal Src kinase
- LSK
- LSTRA
- Lymphocyte cell-specific protein-tyrosine kinase
- lymphocyte-specific protein tyrosine kinase
- p56(LSTRA) protein-tyrosine kinase
- p56lck
- p56-LCK
- pp58lck
- Protein YT16
- Proto-oncogene Lck
- proto-oncogene tyrosine-protein kinase LCK
- T cell-specific protein-tyrosine kinase
- T-lymphocyte specific protein tyrosine kinase p56lck
- tyrosine-protein kinase Lck
- YT16
Background
Lck(lymphocyte-specific protein tyrosine kinase), with 509-amino acid protein (about 56kDa), belongs to the Src non-receptor tyrosine kinases family. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Lck is expressed predominantly in T cells and is localized to the inner surface of the plasma membrane. Lck is activated after T cell stimulation and is required for T-cell proliferation and IL-2 production. Aberrant expression or activation of Lck kinase has been reported in both lymphoid and nonlymphoid malignancies. In addition, inhibition of Lck has been a target to prevent lymphocyte activation and acute rejection.