Product: Heparin (sodium salt)
His Tag Antibody (2A8) Summary
| Immunogen |
6x His synthetic peptide coupled to KLH
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| Specificity |
His-Tag (2A8) Mouse Monoclonal Antibody recognizes over-expressed or recombinant proteins containing the 6x His epitope tag.
|
| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
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Reactivity Notes
6-His Tagged Proteins
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.4)
|
| Preservative |
0.05% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for His Tag Antibody (2A8)
- 6 His epitope tag
- 6X His
- 6X-His
- H
- HHHHHH epitope tag
- HHHHHH tag
- His Tag
- HIS
- polyHistidine
- Poly-histidine
Background
Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (6xHis) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site. The antibody is useful for detection, isolation and localization of the His-tag proteins.