PDE5A Antibody Summary
| Immunogen |
Synthetic cyclic peptide (common to all PDE5A variants) (Rat).
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| Localization |
Located mostly to soluble cellular fractions, and sometimes to particulate cellular fractions.
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| Specificity |
This detects 99 and 89 kDa PDE5A proteins in various tissues.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
PDE5A
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody can be used in Immunoprecipitation and Western Blot. In Western Blot a 89 and 99 kDa bands are seen (predicted MW is 105kDa). Recommended Starting Dilutions*: IP: 1/250 WB: 1/500 Not tested in other applications. *Optimal dilutions should be determined by the end user.
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| Positive Control |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
Tris/Glycine buffer pH 7.5-7.9, stabilizing protein, and glycerol
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| Preservative |
0.02% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for PDE5A Antibody
- CGB-PDE
- cGMP-binding cGMP-specific 3-5-cyclic nucleotide phosphodiesterase
- cGMP-binding cGMP-specific phosphodiesterase
- cGMP-specific 3-5-cyclic phosphodiesterase
- cGMP-specific phosphodiesterase type 5A
- CN5A
- EC 3.1.4
- EC 3.1.4.35
- PDE5
- phosphodiesterase 5A, cGMP-specific
- phosphodiesterase isozyme 5
Background
The cyclic monophosphate nucleotides (cyclic adenosine monophosphate [cAMP] and cyclic guanosine monophosphate [cGMP]) are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A. The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP-dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB-PDEs). The cGB-PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimmeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site. The cGMP-specific phosphodiesterase type-5A (PDE5A) family is comprised of 2 genes (PDE5A and PDE5B) each with multiple splice variants generated by RNA splicing and use of alternate initiation sites. The PDE5 is highly expressed in aorta and lungs, intestine, kidney adrenal gland cerebellum and cerebrum. In cerebellum the PDE5 is highly expressed during neonatal development in Purkinge cells layer. The PDE5 is also abundant in vascular smooth muscle regulating cGMP levels and vascular smooth muscle tonicity. In corpus cavernosum inhibition of PDE5 by sildenafil corrects erectile dysfunctions. The nitric oxide donor sodium notropurside (SNP) stimulate PDE5 activity by cGMP-dependent kinase phosphorylation (Rybalkin et. al., 2002; Murthy 2001). PDE5A1 has 875 amino acids (99.5 kDa). The amino terminal 142 amino acid of the PDE5 gene showed no sequence homology with other PDEs and also contained serine 92 that is phosphorylated by cGMP-kinases (McAllister et al., 93).