CD40/TNFRSF5 Antibody (5C3) Summary
| Immunogen |
The immunogen for this antibody was CD40.
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| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
CD40
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| Purity |
Protein A or G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The 5C3 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test. This antibody may also be used for immunohistochemical staining of frozen tissue sections. 5C3 has also been reported in in vitro functional studies.
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Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
|
| Buffer |
Aqueous, may contain carrier protein/stabilizer.
|
| Preservative |
0.09% Sodium Azide
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| Concentration |
0.5 mg/ml
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| Purity |
Protein A or G purified
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Alternate Names for CD40/TNFRSF5 Antibody (5C3)
- B cell surface antigen CD40
- B-cell surface antigen CD40
- Bp50B cell-associated molecule
- CD40 antigen
- CD40 molecule, TNF receptor superfamily member 5
- CD40 type II isoform
- CD40
- CD40L receptor
- CDw40
- MGC9013
- nerve growth factor receptor-related B-lymphocyte activation molecule
- p50
- TNFRSF5
- TNFRSF5CD40 antigen (TNF receptor superfamily member 5)
- tumor necrosis factor receptor superfamily member 5
- tumor necrosis factor receptor superfamily, member 5
Background
The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.