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Mouse IgG1 isotype control, monoclonal antibody (MOPC-21) (DyLight™ 488 conjugate)
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Isotype matched controls are useful in flow cytometry to determine a gross estimate of non-specific binding that may be observed with a specific antibody of interest.{{69227-93-6} MedChemExpress|{69227-93-6} Protocol|{69227-93-6} Formula|{69227-93-6} manufacturer} In general, an isotype control should be of the same species and isotype as the antibody of interest, used at the same concentration, and conjugated to the same fluorophore. This reagent is an IgG1 isotype mouse monoclonal antibody (Clone # MOPC-21), conjugated to DyLight™-488. It is intended for use as an isotype control antibody for flow cytometry. Flow cytometry analysis of 106 Jurkat cells using the Isotype Control for IgG1 488 compared with a Calreticulin, mAb , at a concentration of 50µg/ml.{{2123481-48-9} MedChemExpress|{2123481-48-9} Biological Activity|{2123481-48-9} Purity|{2123481-48-9} custom synthesis} Flow cytometry analysis of 106 Jurkat cells using the Isotype Control for IgG1 488 compared with a Calreticulin, mAb , at a concentration of 50µg/ml.PMID:31082090
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| Application Flow Cytometry | Clone MOPC-21 | Formulation Liquid. In PBS, pH 7.2, containing 0.09% sodium azide. | Host Mouse | Isotype IgG1 | Source Purified from ascites. | Species Reactivity Species independent | Unit of Measure (UM) µg