Product Name :
Anti-GFP, AlpSdAbs® VHH(iFluor594)
Applications:
ELISA,ICC/IF,Flow Cyt
Reactivity :
GFP
Conjugate:
iFluor594
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-GFP, AlpSdAbs® VHH(iFluor594) is designed for detecting GFP fusion proteins. Anti-GFP, AlpSdAbs® VHH(iFluor594) is based on monoclonal, recombinant, single domain antibody to GFP coupled to iFluor594 . Based on immunoelectrophoresis and/or ELISA, Anti-GFP, AlpSdAbs® VHH(iFluor594) detects the GFP selectively, no reactivity with other proteins. | Immunogen: GFP | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: iFluor594(Ex: 592nm, Em: 614nm), 1 moles iFluor594 per mole VHH | Specificity: GFP | Cross-Reactivity: Recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants.{{1610878-71-1} web|{1610878-71-1} Biological Activity|{1610878-71-1} In stock|{1610878-71-1} manufacturer} Does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Formation: Liquid, 10mM PBS (pH 7.{{1219168-18-9} medchemexpress|{1219168-18-9} Protocol|{1219168-18-9} Data Sheet|{1219168-18-9} manufacturer} 5), 0.PMID:28613653 05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol | Storage: Store at –20 °C(Avoid freeze / thaw cycles), protect from light | Background:Green fluorescent proteins (GFPs) and the variants thereof are widely used to study protein function and location. GFP was originally derived from the jellyfish Aequorea victoria. It has 238 amino acid residues and a green fluorophore, which is comprised of only three amino acids: Ser65-Tyr66-Gly67. The stable protein structure is formed by beta sheets, which have a conformation that makes up an 11-stranded drum-like structure. The stability of GFP allows it to withstand pH levels ranging from mildly acidic (pH=5.5) to extremely basic (pH=12), and can also resist temperatures of up to 65°C. GFP has major and minor excitation peaks at wavelengths of 395 nm and 475 nm, respectively.VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(
Description2 :
Anti-GFP, AlpSdAbs® VHH(iFluor594) is designed for detecting GFP fusion proteins. Anti-GFP, AlpSdAbs® VHH(iFluor594) is based on monoclonal, recombinant, single domain antibody to GFP coupled to iFluor594 . Based on immunoelectrophoresis and/or ELISA, Anti-GFP, AlpSdAbs® VHH(iFluor594) detects the GFP selectively, no reactivity with other proteins.
Immunogen:
GFP
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
Storage :
Store at –20 °C(Avoid freeze / thaw cycles), protect from light
Function:
ELISA: 1:5000 -1:20000Flow Cyt: 1:200-1:2000ICC/IF: 1:200-1:2000Super-resolution microscopyDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.