Product Name :
Acridine Orange hydrochloride
Description:
Acridine Orange hydrochloride is a cell-permeable fluorescent dye that binds to nucleic acids, resulting in an altered spectral emission.
CAS:
65-61-2
Molecular Weight:
301.81
Formula:
C17H20ClN3
Chemical Name:
N3,N3,N6,N6-tetramethylacridine-3,6-diamine hydrochloride
Smiles :
Cl.CN(C)C1=CC2=NC3=CC(=CC=C3C=C2C=C1)N(C)C
InChiKey:
VSTHNGLPHBTRMB-UHFFFAOYSA-N
InChi :
InChI=1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H
Purity:
≥98% (or refer to the Certificate of Analysis)
Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition :
Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.{{Pyrotinib} web|{Pyrotinib} Protein Tyrosine Kinase/RTK|{Pyrotinib} Protocol|{Pyrotinib} In Vitro|{Pyrotinib} custom synthesis|{Pyrotinib} Epigenetics}
Shelf Life:
≥12 months if stored properly.
Stock Solution Storage:
0 – 4 oC for 1 month or refer to the Certificate of Analysis.{{Methotrexate} medchemexpress|{Methotrexate} Cell Cycle/DNA Damage|{Methotrexate} Technical Information|{Methotrexate} In Vitro|{Methotrexate} supplier|{Methotrexate} Autophagy}
Additional information:
Acridine Orange hydrochloride is a cell-permeable fluorescent dye that binds to nucleic acids, resulting in an altered spectral emission.PMID:23819239 |Product information|CAS Number: 65-61-2|Molecular Weight: 301.81|Formula: C17H20ClN3|Chemical Name: N3,N3,N6,N6-tetramethylacridine-3,6-diamine hydrochloride|Smiles: Cl.CN(C)C1=CC2=NC3=CC(=CC=C3C=C2C=C1)N(C)C|InChiKey: VSTHNGLPHBTRMB-UHFFFAOYSA-N|InChi: InChI=1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H|Technical Data|Appearance: Solid Power|Purity: ≥98% (or refer to the Certificate of Analysis)|Solubility: DMSO : 25 mg/mL (82.83 mM; Need ultrasonic). H2O : 50 mg/mL (165.67 mM; ultrasonic and heat to 60°C).|Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis|Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.|Shelf Life: ≥12 months if stored properly.|Stock Solution Storage: 0 – 4 oC for 1 month or refer to the Certificate of Analysis.|Drug Formulation: To be determined|HS Tariff Code: 382200|How to use|In Vitro:|Acridine Orange has been employed extensively as a cytochemical stain and has shown to stain differentially DNA and RNA, and double-restranded nucleic acids in situ. Acridine Orange can either intercalate into double helical nucleic acids (green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (red fluorescence at 640 nm). This unique characteristic makes acridine orange useful for cell-cycle studies. Acridine Orange staining of unfixed cells may be used as a simple, fast means of obtaining information on cell ploidy levels and cell cycle status from DNA measurements (green fluorescence), and cell transcriptional activity from RNA staining (red fluorescence), in human and murine cells lines, peripheral blood and bone marrow specimens from patients with leukemia and mitogenically (phytohemagglutinin) or antigenically (mixed lymphocyte culture) stimulated human peripheral blood cultures.|Products are for research use only. Not for human use.|