E biosynthesis Histidine biosynthesis genes in C. glutamicum Corynebacterium glutamicum strain AS019, a derivative of C. glutamicum ATCC 13059, was used for the initial genetic research on histidine biosynthesis. The genes hisA, encoding the 1-(5-phosphoribosyl)-5-[(5phosphoribosylamino)methylideneamino]imidazole-4 carboxamide (5ProFAR) isomerase, and hisF, encoding 1 subunit from the imidazole glycerol phosphate synthase, were identified by complementation of corresponding histidine auxotrophic E. coli mutants (Jung et al., 1998). The gene hisH, coding for the second subunit of imidazole glycerol phosphate synthase (Kim and Lee, 2001), and the genes hisG and hisE, coding for the ATP2013 The Authors. Microbial Biotechnology published by John Wiley Sons Ltd and Society for Applied Microbiology, Microbial Biotechnology, 7, 5Histidine in C. glutamicumFig. 1. Histidine biosynthetic pathway in C. glutamicum. PRPP, phosphoribosyl pyrophosphate; ATP; adenosine triphosphate; PPi, pyrophosphate; PR-ATP, phosphoribosyl-ATP; PR-AMP, phosphoribosyl-AMP; 5ProFAR, 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino) methylideneamino] imidazole-4 carboxamide; PRFAR, 5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl) imidazole-4-carboxamide; IGP, imidazole-glycerol phosphate; AICAR, 1-(5-phosphoribosyl)-5-amino-4-imidazolecarboxamide; IAP, imidazole-acetol phosphate; Hol-P, L-histidinol phosphate; Pi, phosphate; NAD+, oxidized nicotinamide adenine dinucleotide; NADH, lowered nicotinamide adenine dinucleotide; HisG, ATP phosphoribosyltransferase; HisE, phosphoribosyl-ATP pyrophosphatase; HisI, phosphoribosyl-AMP cyclohydrolase; HisA, 5ProFAR isomerase; HisF, synthase subunit of IGP synthase; HisH, glutaminase subunit of IGP synthase; HisB, imidazoleglycerol-phosphate dehydratase; HisC, histidinol-phosphate aminotransferase; HisN, histidinol-phosphate phosphatase; HisD, histidinol dehydrogenase.2013 The Authors. Microbial Biotechnology published by John Wiley Sons Ltd and Society for Applied Microbiology, Microbial Biotechnology, 7, 5R. K. Kulis-Horn, M. Persicke and J. KalinowskiTable 1. Histidine biosynthesis genes in C. glutamicum ATCC 13032 and the effect of gene disruption on histidine-dependent growth in minimal medium. Gene hisG hisE hisI hisA hisF hisH hisB hisC hisN hisD cg0911 impA cg2301 cg1305 Function ATP phosphoribosyltransferase phosphoribosyl-ATP pyrophosphatase phosphoribosyl-AMP cyclohydrolase 5ProFAR isomerase synthase subunit of IGP synthase glutaminase subunit of IGP synthase imidazoleglycerol-phosphate dehydratase histidinol-phosphate aminotransferase histidinol-phosphate phosphatase histidinol dehydrogenase putative inositol monophosphatase putative inositol monophosphatase putative antibiotic efflux permease, MFS-type L-phenylalanine transporter Effect of gene disruption auxotrophy auxotrophy auxotrophy auxotrophy auxotrophy no effect auxotrophy auxotrophy auxotrophya auxotrophy no impact no effect no effect no effectb References (1), (1), (1), (1), (1), (three) (1), (three) (1), (1), (three) (3) (three) (3), (2), (three) (3) (3) (3) (three) (three) (3)a (three)(4)a.Chenodeoxycholic Acid Development strongly decreased but nonetheless measurable (this study).Rivastigmine b.PMID:23319057 No growth of a Dcg1305 DhisG double mutant in histidine supplemented medium (this study). (1) Mormann et al. (2006): transposon insertion. (two) Zhang et al. (2012): substitution of hisG three end with a chloramphenicol resistance gene. (three) This study: in frame gene deletion. (four) Zhao et al. (2011): in frame gene deletion.
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