S will, even so, let for any randomized phase II study to take place. In conclusion, we have been capable to induce a GSC-specific immune response with out eliciting significant adverse reactions. Our results help the CSC hypothesis and indicate that targeting the CSC population might be therapeutically rewarding. The use of sphere-forming capability for the propagation and enrichment of CSCs is well-established in the glioblastoma. The technology for enriching such cells is transferable to many different tumors; for that reason, the immunotherapy protocol presented right here may possibly be made use of as a model for targeting CSCs in other strong tumors.1508 Acknowledgments We thank the individuals, their households, and all institutions involved in this study. We also thank illustrator Kent Ekstrom for assist with Fig. 1. This perform was supported by the SouthEastern Norway Regional Overall health Authority (#2009024), the Norwegian Research Council by way of the Cancer Stem Cell Innovation Center, plus the National Stem Cell Center.3-Aminobenzamide supplier Conflict of interest None to declare.Cancer Immunol Immunother (2013) 62:1499509 14. Quintana E, Shackleton M, Sabel MS, Fullen DR, Johnson TM, Morrison SJ (2008) Effective tumour formation by single human melanoma cells. Nature 456:59398 15. Eppert K, Takenaka K, Lechman ER, Waldron L, Nilsson B, van Galen P et al (2011) Stem cell gene expression applications influence clinical outcome in human leukemia. Nat Med 17: 1086093 16. Gentles AJ, Plevritis SK, Majeti R, Alizadeh AA (2010) Association of a leukemic stem cell gene expression signature with clinical outcomes in acute myeloid leukemia. JAMA 304: 2706715 17. Herrera MB, Bruno S, Buttiglieri S, Tetta C, Gatti S, Dereqibus MC et al (2006) Isolation and characterization of a stem cell population from adult human liver. Stem Cells 24:2840850 18. Takamiya M, Haider KH, Ashraf M (2011) Identification and characterization of a novel multipotent sub-population of Sca1() cardiac progenitor cells for myocardial regeneration. PLoS A single 6:e25265 19. Wu Y, Wu PY (2009) CD133 as a marker for cancer stem cells: progresses and concerns. Stem Cells Dev 18:1127134 20. Kyte JA, Mu L, Aamdal S, Kvalheim G, Dueland S, Hauser M et al (2006) Phase I/II trial of melanoma therapy with dendritic cells transfected with autologous tumor-mRNA. Cancer Gene Ther 13:90518 21. Mu LJ, Kyte JA, Kvalheim G, Aamdal S, Dueland S, Hauser M et al (2005) Immunotherapy with allotumour mRNA-transfected dendritic cells in androgen-resistant prostate cancer patients.Tempo Epigenetic Reader Domain Br J Cancer 93:74956 22. Brown CE, Starr R, Martinez C, Aquilar B, D’Apuzzo M, Todorov I et al (2009) Recognition and killing of brain tumor stem-like initiating cells by CD8 cytolytic T cells. Cancer Res 69:8886893 23. Pellegatta S, Poliani PL, Corno D, Menghi F, Ghielmetti F, Suarez-Merino B et al (2006) Neurospheres enriched in cancer stem-like cells are very productive in eliciting a dendritic cellmediated immune response against malignant gliomas.PMID:24187611 Cancer Res 66:102470252 24. Saeboe-Larssen S, Fossberg E, Gaudernack G (2002) mRNAbased electrotransfection of human dendritic cells and induction of cytotoxic T lymphocyte responses against the telomerase catalytic subunit (hTERT). J Immunol Strategies 259:19103 25. Suso EM, Dueland S, Rasmussen AM, Vetrhus T, Aamdal S, Kvalheim G et al (2011) hTERT mRNA dendritic cell vaccination: total response inside a pancreatic cancer patient connected with response against several hTERT epitopes. Cancer Immunol Immunother 60:80918 26. Helseth R, Helseth E, Jo.
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