Tients with diabetes. Procedures: Patients at Concord Hospital with suspected CAD gave written informed consent

Tients with diabetes. Procedures: Patients at Concord Hospital with suspected CAD gave written informed consent and have been administered RIPC (sphygmomanometer around the arm, 3 five min cycles, n = 31) or sham (n = 29) before angiography, with recruitment ongoing. Blood was collected pre- and straight away post-RIPC/sham and plateletfree plasma generated. Global coagulation/fibrinolytic possible was measured by overall haemostatic potential assay (Reddel et al. Thromb Res. 2013; 131(5): 457462) and different fibrinolytic aspects by ELISA. EV wereUniversity College Dublin, Dublin, Ireland; bQueen Mary University of London, London, UK; cThe Mater Misericordiae University Hospital, Dublin, Ireland; dWilliam Harvey Investigation institute, Queen Mary University of London, London, UKIntroduction: Urinary extracellular vesicles (uEVs) (exosomes, microvesicles and apoptotic bodies) have potential as diagnostic and prognostic biomarkers. In atherosclerosis, the underlying lead to of heart attack and stroke, EV release might be dysregulated and their contents can mediate pro-inflammatory effects. Many markers have already been previously identified on uEV including exosome markers CD63 and CD9, CD45 (leukocyte marker), CD61 (platelet marker), CD14 (monocyte/macrophage marker) and / integrins. The selectively packaged cargo of these membrane bound carriers include things like microRNAs (miRs). miR-21 and miR-155 are crucial regulatory miRs which might be upregulated in immune cells and are released in EVs following exposure to pro-inflammatory stimuli. miR-155 has been reported to have pro-atherogenic effects and miR-155 deficiency in murine models results in decreased atherosclerotic lesion burden.ISEV2019 ABSTRACT BOOKMethods: Urine was collected from sufferers diagnosed with coronary artery disease (CAD), classified as symptomatic (non-ST-elevation myocardial infarction, STelevation myocardial infarction or unstable angina) or asymptomatic (steady angina). uEVs from symptomatic and asymptomatic sufferers have been isolated through benchtop centrifugation. The concentration and size of uEVs had been analysed through the NanoSight NS300 (n = 15 per group). The expression of miR-155 and miR-21 was investigated by RT-qPCR (n = ten per group). uEV FCGR2A/CD32a Proteins site surface marker expression was analysed by ImageStreamX MK2 Imaging Flow Cytometer (12 per group). Final results: uEV concentration in symptomatic individuals (IgG2B Proteins Source median; 6.46E+9 particles/mL) was significantly decreased (p 0.05) in comparison to asymptomatic sufferers (median; 1.25E+10 particles/mL). CD11B+ uEVs were increased and CD16+ uEVs had been decreased within the symptomatic individuals (p 0.01). Furthermore, the concentration of CD45+ EVs have been increased in symptomatic patients (p 0.001). Despite the fact that uEV miR-21 was unchanged, miR-155 expression was considerably elevated inside the symptomatic group (p 0.05). Summary/Conclusion: uEV concentration, miR-155 expression and surface marker expression have diagnostic and prognostic possible. As CAD severity increases, uEV concentration is decreased, surface marker expression is altered and uEV miR-155 expression is improved. Funding: The Irish Analysis Council.OT01.Circulating extracellular vesicle-associated microRNAs as predictive biomarkers of cardiovascular complications in end-stage renal disease Dakota D. Gustafsona, Jessica Fitzpatrickb, Jason Fishc and Rulan Parekhba Division of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; bChild Health Evaluative Sciences, Research Institute, The Hospital for Sick Youngsters,.