p97/VCP Antibody (Hs-14) [FITC] Summary
| Immunogen |
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
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| Specificity |
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name “intra-acrosomal protein”.
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| Isotype |
IgM
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
VCP
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| Purity |
Size Exclusion Chromatography
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The reagent is designed for Flow Cytometry analysis of sperm cells using 20 ul reagent / 100 ul cell suspension.
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| Publications |
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
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| Buffer |
TBS and 0.2% (w/v) high grade BSA (protease free)
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| Preservative |
15mM Sodium Azide
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| Purity |
Size Exclusion Chromatography
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Alternate Names for p97/VCP Antibody (Hs-14) [FITC]
- 15S Mg(2+)-ATPase p97 subunit
- IBMPFD
- IBMPFDMGC8560
- MGC131997
- p97
- TER ATPase
- TERA
- transitional endoplasmic reticulum ATPase
- valosin containing protein
- Valosin-containing proteinMGC148092
- VCP
- yeast Cdc48p homolog
Background
VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.