p97/VCP Antibody (Hs-14) [Alexa Fluor® 405] Summary
| Immunogen |
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
|
| Specificity |
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa protein previously identified under the general name “intra-acrosomal protein”.
|
| Isotype |
IgM
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
VCP
|
| Purity |
Size Exclusion Chromatography
|
| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
ICC: Use at an assay dependant dilution. Designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought). Membrane permeabilization with acetone is essential. WB: Use at an assay dependant dilution. Detects a band of approximately 220 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
|
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
|
| Buffer |
50mM Sodium Borate
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Size Exclusion Chromatography
|
Alternate Names for p97/VCP Antibody (Hs-14) [Alexa Fluor® 405]
- 15S Mg(2+)-ATPase p97 subunit
- IBMPFD
- IBMPFDMGC8560
- MGC131997
- p97
- TER ATPase
- TERA
- transitional endoplasmic reticulum ATPase
- valosin containing protein
- Valosin-containing proteinMGC148092
- VCP
- yeast Cdc48p homolog