p53 Antibody (TRP/817) – IHC-Prediluted Summary
| Immunogen |
Recombinant human TP53 protein
|
| Localization |
Predominantly nuclear
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| Specificity |
Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 protein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
|
| Isotype |
IgG2b Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
TP53
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
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| Application Notes |
Hu-chromosome location: 17p13.1 Immunohistochemistry-Paraffin 0.25 – 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for p53 Antibody (TRP/817) – IHC-Prediluted
- Antigen NY-CO-13
- BCC7
- FLJ92943
- LFS1
- LFS1TRP53
- p53 tumor suppressor
- p53
- P53cellular tumor antigen p53
- Phosphoprotein p53
- TP53
- transformation-related protein 53
- TRP53
- tumor protein p53
- Tumor suppressor p53