p21/CIP1/CDKN1A Antibody (SPM306) – IHC-Prediluted Summary
| Immunogen |
Human recombinant p21 protein
|
| Localization |
Nuclear
|
| Specificity |
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
|
| Isotype |
IgG2a Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
CDKN1A
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Hu-chromosome location: 6p21.31 Immunohistochemistry-Paraffin 2 – 4 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS and 0.05% BSA
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A or G purified
|
Alternate Names for p21/CIP1/CDKN1A Antibody (SPM306) – IHC-Prediluted
- CAP20cyclin-dependent kinase inhibitor 1
- CDK-interacting protein 1
- CDKN1A
- CDKN1melanoma differentiation associated protein 6
- CIP1
- CIP1WAF1CDK-interaction protein 1
- cyclin-dependent kinase inhibitor 1A (p21, Cip1)
- MDA6
- MDA-6
- Melanoma differentiation-associated protein 6
- p21
- p21CIP1
- p21Cip1/Waf1
- PIC1
- SDI1DNA synthesis inhibitor
- wild-type p53-activated fragment 1