nNOS Antibody Summary
| Immunogen |
Synthetic peptide corresponding to the sequence near the C-terminus of human nNOS.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
NOS1
|
| Purity |
Protein A purified
|
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
This antibody is useful in Western Blot (Detects a band of ~155kDa).
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Reactivity Notes
Recognizes Mouse and Rat nNOS.
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.2) and 50% Glycerol
|
| Preservative |
0.09% Sodium Azide
|
| Purity |
Protein A purified
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Alternate Names for nNOS Antibody
- bNOS
- brain
- Constitutive NOS
- EC 1.14.13.39
- IHPS1
- Neuronal NOS
- nitric oxide synthase 1 (neuronal)
- nNOS
- N-NOS
- nNOSNOS type I
- NOS
- NOS1
- Peptidyl-cysteine S-nitrosylase NOS1
Background
The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.