hnRNP C1 C2 Antibody (4F4) Summary
| Immunogen |
RNP eluted from oligo (dT) cellulose column
|
| Localization |
Nucleus
|
| Specificity |
This detects a band at approximately 41 and 43kDa.
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
HNRNPC
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
In Western Blot a 41kDa (C1) and 43kDa 9C3) bands are seen. Not tested in other applications. *Optimal dilutions should be determined by the end user.
|
Reactivity Notes
Cross-reacts with Human, Chicken and Monkey. May also react with Hamster.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
10mM PBS (pH 7.4)
|
| Preservative |
15mM Sodium Azide
|
| Concentration |
2.0 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for hnRNP C1 C2 Antibody (4F4)
- C1
- C2
- heterogeneous nuclear ribonucleoprotein C (C1/C2)
- heterogeneous nuclear ribonucleoproteins C1/C2
- hnRNP C1/C2
- HNRNP
- hnRNPC
- HNRPC
- MGC104306
- MGC105117
- MGC117353
- MGC131677
- nuclear ribonucleoprotein particle C1 protein
- nuclear ribonucleoprotein particle C2 protein
- SNRPC
Background
RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either byimmunoprecipitation or by sucrose gradient fractionation of cell extracts. When this is performed, the hnRNPs are isolated (consisting of protein groups named A to U), and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are theA1, A2, B1, B2, C1, and C2 with a range of molecular weight starting with 34 kDa up to 43 kDa. The hnRNP-C proteins have a single RNP motif RNA-binding domain (RBD) of 80 to 100 amino acidlong. The hnRNP -C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding.4 Although its physiological action is unknown, mutation of the hnRNPC gene causes a embryonic lethal phenotype.