beta II Tubulin B Antibody (5B2) Summary
| Immunogen |
Recombinant human beta-Tubulin (1-445aa) purified from E. coli
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| Specificity |
Anti-human beta-Tubulin mAb, clone 5B2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human beta-Tubulin protein.
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| Isotype |
IgG2b Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
TUBB2B
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| Purity |
Protein G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:1000 ~ 5000.Recommended starting dilution is 1:1000
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS (pH 7.4) and 10% Glycerol
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Protein G purified
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Alternate Names for beta II Tubulin B Antibody (5B2)
- bA506K6.1
- class II beta-tubulin isotype
- DKFZp566F223
- FLJ98847
- MGC8685
- tubulin beta-2B chain
- tubulin, beta 2B
- tubulin, beta polypeptide paralog
Background
Tubulin is a major cytoskeleton component that has five distinct forms. Multiple beta Tubulin isoforms have been characterized and are expressed in mammalian tissues. Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.