beta-Catenin Antibody (8E7) – Active Summary
| Immunogen |
Fusion protein corresponding to residues 1-100 of human beta-catenin with a c-terminal histidine tag
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| Epitope |
The epitope corresponds to amino acid residues 36-44 (HSGATTTAP) of human beta-catenin.
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| Marker |
Epithelial Cell Marker, Adherens Junction Marker
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| Specificity |
Recognizes active form of beta-catenin, dephosphorylated on Ser37 or Thr41.
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| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
CTNNB1
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| Purity |
Protein G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody is useful in Flow Cytometry, Immunocytochemistry, Immunohistochemistry-Paraffin, Western Blot. In Immunocytochemistry this antibody showed positive staining on the membrane and cytosol.
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Reactivity Notes
Human, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
0.1M Tris-Glycine (phH 7.4), 0.15M NaCl and 30% Glycerol
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| Preservative |
0.05% Sodium Azide
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| Purity |
Protein G purified
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Alternate Names for beta-Catenin Antibody (8E7) – Active
- bCatenin
- beta 1 (88kD)
- beta-Catenin
- catenin (cadherin-associated protein), beta 1, 88kDa
- catenin beta-1
- CTNNB
- CTNNB1
- DKFZp686D02253
- FLJ25606
- FLJ37923
Background
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as a-catenin and beta-catenin. a-catenin can bind to beta-catenin and can also bind actin. beta-catenin binds the cytoplasmic domain of some cadherins. When beta-catenin was sequenced it was found to be a member of the armadillo family of proteins. These proteins have multiple copies of the so-called armadillo repeat domain which is specialized for protein-protein binding. An increase in beta-catenin production has been noted in those people who have Basal Cell Carcinoma and leads to the increase in proliferation of related tumors. When beta-catenin is not associated with cadherins and a-catenin, it can interact with other proteins such as Catenin Beta Interacting Protein 1 (ICAT) and adenomatosis polyposis coli (APC). Recent evidence suggests that beta-catenin plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.