beta-Actin Antibody Summary
| Immunogen |
Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beta-actin. The immunogen is located within the last 50 amino acids of Beta-actin.
|
| Clonality |
Polyclonal
|
| Host |
Rabbit
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| Gene |
ACTB
|
| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
b-actin antibody can be used for the detection of b-actin by Western blot at 1 – 2 mg/ml.
|
| Theoretical MW |
41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for beta-Actin Antibody
- ACTB
- actin, beta
- actin, cytoplasmic 1
- Beta Actin
- beta cytoskeletal actin
- betaActin
- beta-Actin
- BRWS1
- PS1TP5-binding protein 1
- PS1TP5BP1
Background
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.