alpha-Fetoprotein/AFP Antibody (C2) – Azide and BSA Free Summary
| Immunogen |
Alpha fetoprotein (AFP) purified from serum of a hepatoma patient.
|
| Localization |
Cytoplasmic
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| Marker |
Hepatocellular/Germ Cell Tumor Marker
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| Specificity |
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
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| Isotype |
IgG1 Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
AFP
|
| Purity |
Protein A or G purified
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|
Applications/Dilutions
| Dilutions |
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| Application Notes |
Hu-chromosome location: 4q13.3 Immunohistochemistry-Paraffin 1 – 2 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS
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| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
Protein A or G purified
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Alternate Names for alpha-Fetoprotein/AFP Antibody (C2) – Azide and BSA Free
- AFP
- Alpha-1-fetoprotein
- alpha-fetoglobulin
- alpha-Fetoprotein
- DSCAM2
- FETA
- HP
- HPAFP