alpha-Fetoprotein/AFP Antibody (C2)

Product: Cyclobenzaprine (hydrochloride)

alpha-Fetoprotein/AFP Antibody (C2) Summary

Immunogen
Alpha fetoprotein (AFP) purified from serum of a hepatoma patient.
Localization
Cytoplasmic
Marker
Hepatocellular/Germ Cell Tumor Marker
Specificity
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP) (ISOBM TD-2 workshop). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AFP
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 – 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 1 – 2 ug/ml
  • Immunofluorescence 0.5 – 1.0 ug/ml
Application Notes
Hu-chromosome location: 4q13.3
Immunohistochemistry-Paraffin 1 – 2 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for alpha-Fetoprotein/AFP Antibody (C2)

  • AFP
  • Alpha-1-fetoprotein
  • alpha-fetoglobulin
  • alpha-Fetoprotein
  • DSCAM2
  • FETA
  • HP
  • HPAFP

PMID: 9514305