alpha COP I Antibody Summary
| Immunogen |
Synthetic peptide corresponding to residues M(1) L T K F E T K S A R V K G L S F H P(19) C of rat alpha-COP.
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| Localization |
Cytoplasmic and Golgi Apparatus
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| Specificity |
Detects coatomer-protein I alpha (COP I alpha).
|
| Isotype |
IgG
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
COPA
|
| Purity |
Immunogen affinity purified
|
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|
Applications/Dilutions
| Dilutions |
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| Application Notes |
WB: Detects an approx. 140 kDa protein representing COPI alpha from PC-12 cell extract. Also detects an unidentified protein at approx. 100 kDa. ICC: Staining of alpha COP I results in alpha COP localization to the Golgi complex and to distinct vesicular structures scattered throughout the cytoplasm. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
138 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -20C. Avoid freeze-thaw cycles.
|
| Buffer |
PBS, 1mg/ml BSA
|
| Preservative |
0.05% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
|
Alternate Names for alpha COP I Antibody
- alpha coat protein
- Alpha-coat protein
- Alpha-COP
- coatomer protein complex, subunit alpha
- coatomer subunit alpha
- FLJ26320
- HEPCOP
- HEP-COPalpha-COP
- xenin
Background
Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.