VAMP3/Cellubrevin Antibody (4G9) Summary
| Immunogen |
Recombinant human cellubrevin (1-77aa) purified from E. coli
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| Specificity |
Anti-human VAMP3 mAb, clone k2A2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human cellubrevin protein.
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| Isotype |
IgG2b Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
VAMP3
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| Purity |
Protein G purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1,000 ~ 2,000. Recommended starting dilution is 1:1,000. Use in immunoprecipitation reported in scientific literature (PMID 27183589).
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| Publications |
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS (pH 7.4) and 10% Glycerol
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Protein G purified
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Alternate Names for VAMP3/Cellubrevin Antibody (4G9)
- CEBCellubrevin
- cellubrevin
- SYB3
- Synaptobrevin-3
- VAMP-3
- vesicle-associated membrane protein 3 (cellubrevin)
- vesicle-associated membrane protein 3
Background
Cellubrevin, also known as VAMP3, is a member of the Vamp/synaptobrevin family. Vamps, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. Cellubrevin is a vesicular soluble N-ethylmaleimide sensitive factor attachment protein receptor (v-SNARE) homologous to the neuronal synaptobrevins 1/2 and is also a substrate of tetanus neurotoxin.