Ulex Europaeus Lectin 1 Antibody Summary
| Immunogen |
Ulex europaeus agglutinin isolated by affinity chromatography from seeds of Ulex europaeus (gorse seed).
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| Marker |
Endothelial Cells
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| Specificity |
The antibody may be used for studies of the vascular structures. It reacts specifically with UEA-I bound to human endothelial cells of normal and neoplastic blood and lymphatic vessels. Its uniform reaction with vessels enables differentation between vessels and artificial slits in the tissue.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Purity |
Unpurified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Lectin isolated from Ulex europaeus I (NBP2-42700) is specific for alpha-L-fucose-containing glycocompounds which (in combination with NB110-13922) makes it a specific marker for vascular endothelial cells and tumors derived from them. For immunoassays on animal tissues, Ulex Europaeus Lectin 1 Protein (NBP2-42700) would be a required product alongwith NB110-13922. Technically, NBP2-42700 will bind the carbohydrate moiety on vascular endothelial cells as first primary reagent and then NB110-13922 will bind to Ulex Europaeus-1 lectin (NBP2-42700) in the primary antibody incubation step (See product specific IHC-P Protocol).
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| Positive Control |
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Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
Whole antisera
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| Preservative |
15mM Sodium Azide
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| Purity |
Unpurified
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Alternate Names for Ulex Europaeus Lectin 1 Antibody
- Gorse or Furze Lectin
- UEA-1; H(O) lectin 1; Anti H(O) lectin I
Background
Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques. Anti-Ulex Europaeus-I (UEA-I) Lectin is developed in rabbit using purified Ulex Europaeus-I lectin from gorse seeds as the immunogen. Anti-UEA-I Lectin is specific for UEA-I bound to vascular endothelium and squamous epithelium of human tonsil by indirect immunofluorescent staining. While erythrocytes may be stained,no other tonsilar elements are reactive with the antibody. Anti-UEA-I reacts with UEA-I bound to human endothelial cells of normal and neoplastic blood and lymphatic vessels. It also reacts with human epithelia such as in the colon, bronchus, epidermis, sweat gland ducts and hair follicles. Epithelia from other species may also react with the antibody. Anti-Ulex Europaeus-I Lectin may be used for studies of vascular structures in formalin-fixed, paraffin-embedded tissue or frozen tissue preparations. The antibody may be useful in the detection of vascular tumors, the investigation of vascular invasion by tumor cells, for determination of UEA-I binding to normal, embryonal, dysplastic and neoplastic epithelia, and the study of storage diseases such as fucosidosis Ulex Europaeus-I Lectin is a 46 kDa glycoprotein known to interact with alpha-L fucosyl residues in oligosaccharides present on the membranes of human blood group O erythrocytes, human endothelial cells and a variety of human and animal epithelial cells.