Product: para-iodoHoechst 33258
Ubiquitin-activating Enzyme/UBE1 Antibody Summary
| Immunogen |
Recombinant full length UBE1 fusion protein (Human)
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| Specificity |
UBE1
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
UBA1
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| Purity |
IgG purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Expect a band at ~118 kDa in size corresponding – uBE1 by western blotting in the appropriate cell lysate or extract.
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| Positive Control |
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| Publications |
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Reactivity Notes
Reacts with Human. Predicted to react with Mouse (95% identity with immunogen), Rat (95% identity with immunogen), Rabbit (96% identity with immunogen) and Dog (93% identity with immunogen) due to sequence homology. Not yet tested in other species.
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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| Buffer |
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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| Preservative |
0.01% Sodium Azide
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| Purity |
IgG purified
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Alternate Names for Ubiquitin-activating Enzyme/UBE1 Antibody
- A1S9T and BN75 temperature sensitivity complementing
- A1S9T
- A1ST
- AMCX1
- GXP1
- MGC4781
- POC20 centriolar protein homolog
- POC20
- Protein A1S9
- SMAX2
- UBA1
- UBA1, ubiquitin-activating enzyme E1 homolog A
- UBA1A
- UBE1
- UBE1A1S9
- UBE1X
- ubiquitin-activating enzyme E1 (A1S9T and BN75 temperature sensitivitycomplementing)
- Ubiquitin-activating enzyme E1
- Ubiquitinactivating Enzyme
- Ubiquitin-activating Enzyme
- ubiquitin-like modifier activating enzyme 1
- ubiquitin-like modifier-activating enzyme 1
Background
Ubiquitinylation, the covalent attachment of ubiquitin to protein, regulates a number of cellular processes including responses to stress. This process is responsible for selective targeting of proteins for degradation. The first step in ubiquitinylation is the activation of ubiquitin by ubiquitin activating enzyme E1 which is a rate-limiting enzyme in the conjugation process. The ubiquitin activating enzyme exists as two isoforms: E1a (117 kDa), localized predominantly in the nucleus, and E1b (110 kDa), localized in the cytoplasm. The first 11 amino acids are considered to be essential for phosphorylation and exclusive nuclear localization of the enzyme.