UBQLN4/CIP75 Antibody

Product: D-Glutamic acid

UBQLN4/CIP75 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Ubiquilin 4. The exact sequence is proprietary.
Localization
Nucleus; Cytoplasm; Endoplasmic reticulum (Probable)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
UBQLN4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 10 – 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBQLN4/CIP75 Antibody

  • A1Uataxin-1 ubiquitin-like interacting protein
  • Ataxin-1 ubiquitin-like-interacting protein A1U
  • C1orf6ubiquilin-4
  • chromosome 1 open reading frame 6
  • UBINCIP75
  • ubiquilin 4

PMID: 16915381

UBQLN4/CIP75 Antibody

Product: BPR1J-098

UBQLN4/CIP75 Antibody Summary

Immunogen
Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CIP75. The immunogen is located within the first 50 amino acids of CIP75.
Clonality
Polyclonal
Host
Rabbit
Gene
UBQLN4
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
Control Peptide
UBQLN4/CIP75 Peptide (NBP1-77054PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBQLN4/CIP75 Antibody

  • A1Uataxin-1 ubiquitin-like interacting protein
  • Ataxin-1 ubiquitin-like-interacting protein A1U
  • C1orf6ubiquilin-4
  • chromosome 1 open reading frame 6
  • UBINCIP75
  • ubiquilin 4

Background

The ubiquitin-proteosome pathway of protein degradation is one of the mechanisms that ensure the proper level of cellular proteins. The ubiquitin-like (UbL) and ubiquitin-associated (UBA) domain containing protein family is thought to be involved in proteosomal degradation. One such protein is CIP75, also known as ubiquilin-4, interacts with a number of proteins such as Ataxin-1 and Connexin-43, resulting in an increased rate of turnover of these proteins. Overexpression CIP75 led to a significant reduction of Connexin-43 half-life, with the opposite being observed in siRNA knockdown experiments. CIP75 contains an N-terminal UbL domain which is thought to interact with proteins of the 26S proteosome complex and a C-terminal UBA domain which appears to mediate its interaction with Connexin-75. At least three isoforms of CIP75 are known to exist.

PMID: 15897464