Transducin alpha Antibody

Product: Midodrine (hydrochloride)

Transducin alpha Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human GNAT1. The exact sequence is proprietary.
Predicted Species
Rat (98%), Bovine (98%), Guinea Pig (99%), Zebrafish (92%), Canine (99%), Chicken (96%), Xenopus (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNAT1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry-Frozen 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Transducin alpha Antibody

  • CSNBAD3
  • GBT1
  • GNATR
  • guanine nucleotide binding protein (G protein), alpha transducing activitypolypeptide 1
  • guanine nucleotide-binding protein G(t) subunit alpha-1
  • guanine nucleotide-binding protein G(T), alpha-1 subunit
  • rod-type transducin alpha subunit
  • Transducin alpha-1 chain
  • transducin, rod-specific

Background

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

PMID: 10744717

Transducin alpha Antibody

Product: Schisantherin E

Transducin alpha Antibody Summary

Immunogen
Synthetic Peptide: K(18) L K E D A E K D A R T V K(31) C
Localization
Cell membrane.
Specificity
Detects Rod Transducin alpha from bovine outer rod segments and sheep retinal extract.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNAT1
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
WB: Detects an approx. 38 kDa protein representing Rod Transducin alpha from sheep retinal extract.

Reactivity Notes

Expected cross-reactivity with Human base on sequence homology. Mouse reactivity reported in scientific literature ( PMID: 11095744). Porcine reactivity reported in scientific literature ( PMID: 22108793).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Transducin alpha Antibody

  • CSNBAD3
  • GBT1
  • GNATR
  • guanine nucleotide binding protein (G protein), alpha transducing activitypolypeptide 1
  • guanine nucleotide-binding protein G(t) subunit alpha-1
  • guanine nucleotide-binding protein G(T), alpha-1 subunit
  • rod-type transducin alpha subunit
  • Transducin alpha-1 chain
  • transducin, rod-specific

Background

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.

PMID: 20208034