Product: CHIR-99021 (trihydrochloride)
Topoisomerase I Antibody Summary
| Immunogen |
The immunogen for this product maps to a region between residue 100 and 150 of human DNA topoisomerase I using the numbering given in entry NP_003277.1 (GeneID 7150).
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| Predicted Species |
Rat (100%), Porcine (100%), Primate (100%), Bovine (100%), Chinese Hamster (100%), Rhesus Macaque (100%), Rabbit (100%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
TOP1
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| Purity |
Immunogen affinity purified
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| Innovators Reward |
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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| Theoretical MW |
91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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Reactivity Notes
Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee, White-tufted-ear marmoset, Green monkey, Crab-eating macaque, African elephant, Small-eared galago and Northern white-cheeked gibbon.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
TBS and 0.1% BSA
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| Preservative |
0.09% Sodium Azide
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| Concentration |
0.2 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for Topoisomerase I Antibody
- DNA topoisomerase 1
- DNA topoisomerase I
- EC 5.99.1.2
- TOPI
- topoisomerase (DNA) I
- type I DNA topoisomerase
Background
Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.