Thymidylate Synthase Antibody (TS106) – Azide and BSA Free Summary
Immunogen |
Recombinant human thymidylate synthase
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Localization |
Nuclear & Cytoplasmic
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Marker |
5-FU Resistance Marker
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Specificity |
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
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Isotype |
IgG1 Kappa
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Clonality |
Monoclonal
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Host |
Mouse
|
Gene |
TYMS
|
Purity |
Protein A purified
|
Innovators Reward |
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Applications/Dilutions
Dilutions |
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Application Notes |
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Theoretical MW |
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
Buffer |
10mM PBS – BSA free
|
Preservative |
No Preservative
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Concentration |
1.0 mg/ml
|
Purity |
Protein A purified
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Alternate Names for Thymidylate Synthase Antibody (TS106) – Azide and BSA Free
- EC 2.1.1.45
- HsT422
- MGC88736
- thymidylate synthase
- thymidylate synthetase
- TMSTsase
- TSase
- TSHST422