Product: VTP-28000 (Hydrochloride)
Thymidylate Synthase Antibody (SPM453) [Biotin] Summary
| Immunogen |
Recombinant human thymidylate synthase
|
| Localization |
Cytoplasmic
|
| Marker |
5-FU Resistance Marker
|
| Specificity |
It recognizes a protein of 36kDa, identified as Thymidylate Synthase (TS) (EC 2.1.1.45). TS converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5,10-methylenetetrahydrofolate. Expression of TS protein is associated with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas.
|
| Isotype |
IgG1 Kappa
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
TYMS
|
| Purity |
Protein A purified
|
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Applications/Dilutions
| Dilutions |
|
| Application Notes |
Immunohistochemistry (Staining of paraffin tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
|
| Theoretical MW |
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Readout System |
|
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark.
|
| Buffer |
PBS
|
| Preservative |
0.05% Sodium Azide
|
| Purity |
Protein A purified
|
Alternate Names for Thymidylate Synthase Antibody (SPM453) [Biotin]
- EC 2.1.1.45
- HsT422
- MGC88736
- thymidylate synthase
- thymidylate synthetase
- TMSTsase
- TSase
- TSHST422