TOR/mTOR Antibody Summary
| Immunogen |
The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475).
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| Specificity |
This is specific for Mammalian Target of Rapamycin (mTOR).
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| Predicted Species |
Porcine (100%), Primate (100%), Monkey (100%), Guinea Pig (100%), Bovine (100%), Equine (100%), Canine (100%), Sheep (100%), Rabbit (100%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
MTOR
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Western analysis: An overnight incubation at 4 degrees Celcius is recommended for primary antibody use. mTOR is ~235 kDa and optimally requires detergent and extended Western blotting transfer times. For example from 5% acrylamide gels to PVDF: 50V for 180 min. in CAPS buffer with 10% MeOH and 0.1% SDS was found to be optimal. Immunohistochemistry/Immunocytochemistry is not recommended. Please use NB100-240. Nuclear extracts are recommended.
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| Positive Control |
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| Publications |
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Reactivity Notes
Based on 100% sequence identity, this antibody is predicted to react with Panda, Rhesus Monkey, White-tufted-ear marmoset, Crab-eating macaque, European domestic ferret, African elephant, Chinese hamster and Northern white-cheeked gibbon.
Packaging, Storage & Formulations
| Storage |
Store at 4C. Do not freeze.
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| Buffer |
Tris-Citrate/Phosphate (pH 7.0 – 8.0)
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| Preservative |
0.09% Sodium Azide
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| Concentration |
1.0 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for TOR/mTOR Antibody
- EC 2.7.11.1
- FK506 binding protein 12-rapamycin associated protein 1
- FK506 binding protein 12-rapamycin associated protein 2
- FK506-binding protein 12-rapamycin complex-associated protein 1
- FKBP12-rapamycin complex-associated protein
- FLJ44809
- FRAP
- FRAP1
- FRAP1FKBP12-rapamycin complex-associated protein 1
- FRAP2
- Mammalian target of rapamycin
- mechanistic target of rapamycin (serine/threonine kinase)
- Mechanistic target of rapamycin
- MTOR
- RAFT1
- rapamycin and FKBP12 target 1
- rapamycin associated protein FRAP2
- Rapamycin target protein 1
- RAPT1FKBP-rapamycin associated protein
- serine/threonine-protein kinase mTOR
- TOR
Background
FKBP 12 rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 235 kDa protein (symbolized RAFT 1) with significant homology to the Saccharomyces cerevisiae protein TOR 1 and has been shown to associate with the immunophilin FKBP 12 in a rapamycin-dependent fashion. The FKBP 12 rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP 12 rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.