TOP1MT Antibody (TOP1MT/488) – IHC-Prediluted Summary
| Immunogen |
Recombinant full-length human TOP1MT protein
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| Localization |
Cytoplasmic (Mitochondria)
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| Specificity |
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.
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| Isotype |
IgG2b Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
TOP1MT
|
| Purity |
Protein A or G purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
Hu-chromosome location: 8q24.3 Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
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| Buffer |
10mM PBS and 0.05% BSA
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| Preservative |
0.05% Sodium Azide
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| Purity |
Protein A or G purified
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Alternate Names for TOP1MT Antibody (TOP1MT/488) – IHC-Prediluted
- mitochondrial DNA topoisomerase I
- mitochondrial
- topoisomerase (DNA) I, mitochondrial