TIP60 Antibody (3F9)

Product: Stachydrine

TIP60 Antibody (3F9) Summary

Immunogen
Ni-NTA purified truncated recombinant TIP60 expressed in E. Coli strain BL21 (DE3).
Specificity
TIP60 (3F9)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
KAT5
Purity
Unpurified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 – 1:2000
  • ELISA 1:10000
  • Immunohistochemistry 1:10 – 1:500
  • Immunohistochemistry-Paraffin 1:200 – 1:1000
Application Notes
This antibody is useful for: Western Blot: 1:500- 1: 2,000 IHC-P: 1:500- 1:2000 ELISA: Proposed dilution 1:10,000.
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
TIP60 Lysate (NBL1-12124)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for TIP60 Antibody (3F9)

  • cPLA2
  • ESA1EC 2.3.1.48
  • Histone acetyltransferase HTATIP
  • histone acetyltransferase KAT5
  • HIV-1 Tat interactive protein
  • HIV-1 Tat interactive protein, 60kDa
  • HTATIP1
  • HTATIPcPLA2 interacting protein
  • K(lysine) acetyltransferase 5,60 kDa Tat-interactive protein
  • K-acetyltransferase 5
  • Lysine acetyltransferase 5
  • PLIPTIP
  • Tat interacting protein, 60kDa
  • Tip60
  • TIP60cPLA(2)-interacting protein

Background

HTATIP (HIV-1 Tat interacting protein TIP60, about 60kDa) belongs to the MYST family of histone acetyl transferases (HATs) and was originally isolated as an HIV-1 TAT-interactive protein. HATs play important roles in regulating chromatin remodeling, transcription and other nuclear processes by acetylating histone and nonhistone proteins. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. In addition to the growing number of post-translational histone modifications regulating chromatin structure, cells can also exchange canonical histones with variant histones that can directly or indirectly modulate chromatin structure. There are five major variants of histone H2A: canonical H2A (most abundant), H2A.X, MacroH2A, H2ABbd and H2A.Z. Histone H2A.Z, the most conserved variant across species, functions as both a positive and negative regulator of transcription and is important for chromosome stability. Several homologous protein complexes, such as SWR-C, TIP60 and SRCAP (mammals), have been shown to catalyze the ATP-dependent exchange of H2A.Z for H2A in the nucleosome. This protein is a histone acetylase that has a role in DNA repair and apoptosis and is thought to play an important role in signal transduction.

PMID: 24976876