Smad2 Antibody (376520) Summary
| Immunogen |
E. coli-derived recombinant human Smad2
Lys20-Thr108 Accession # Q15796 |
| Specificity |
Detects human Smad2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Smad1, 3, 4, 5, 6, 7, or 8 is observed.
|
| Source |
N/A
|
| Isotype |
IgG2b
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
SMAD2
|
| Purity |
Protein A or G purified from hybridoma culture supernatant
|
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Applications/Dilutions
| Dilutions |
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Packaging, Storage & Formulations
| Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
| Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
|
| Preservative |
No Preservative
|
| Concentration |
LYOPH
|
| Purity |
Protein A or G purified from hybridoma culture supernatant
|
| Reconstitution Instructions |
Reconstitute at 0.5 mg/mL in sterile PBS.
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Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Smad2 Antibody (376520)
- hMAD-2
- hSMAD2
- JV18
- JV18-1
- JV18-1SMAD, mothers against DPP homolog 2 (Drosophila)
- MAD homolog 2
- Mad protein homolog
- Mad, mothers against decapentaplegic homolog 2
- MADH2
- MADH2MGC34440
- MADR2
- MADR2MGC22139
- Mad-related protein 2
- mother against DPP homolog 2
- mothers against decapentaplegic homolog 2
- Mothers against DPP homolog 2
- Sma- and Mad-related protein 2
- SMAD 2
- SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila)
- SMAD, mothers against DPP homolog 2
- Smad2
- Smox
Background
Smad2 is a 52 kDa TGF-beta receptor-regulated member of the Smad family. It is expressed most highly in skeletal muscle, heart and placenta. Receptor binding of TGF-beta causes C-terminal phosphorylation of Smads 2 and 3. Smad is released from cytoplasmic anchoring, complexes with Smad4 and accumulates in the nucleus. After regulating expression, Smad2 is dephosphorylated and recycled. The 467 aa human Smad2 shows only two aa differences with mouse Smad2. About 10% of Smad2 expressed as a short, high-activity isoform missing aa 80‑108 within the MH1 domain.