Sirtuin 3/SIRT3 Antibody

Product: BML-210

Sirtuin 3/SIRT3 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:FSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYT
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SIRT3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:20-1:50
Application Notes
Immunohistochemistry retrieval recommended: HIER pH6 The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
Sirtuin 3/SIRT3 Protein (NBP1-87037PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (85%), Rat (86%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Sirtuin 3/SIRT3 Antibody

  • EC 3.5.1
  • EC 3.5.1.-
  • hSIRT3
  • mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
  • NAD-dependent deacetylase sirtuin-3, mitochondrial
  • SIR2L3
  • SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
  • sir2-like 3
  • SIR2-like protein 3
  • SIRT3
  • sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
  • Sirtuin 3
  • sirtuin type 3

PMID: 8863500

Sirtuin 3/SIRT3 Antibody

Product: Ibutamoren (Mesylate)

Sirtuin 3/SIRT3 Antibody Summary

Immunogen
Peptide with sequence C-QRETGKLDGPDK corresponding to C-Terminus according to NP_036371.1, NP_001017524.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).
Clonality
Polyclonal
Host
Goat
Gene
SIRT3
Purity
Immunogen affinity purified
Innovators Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Learn about the Innovators Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.1 – 0.3 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 28 kDa band observed in human liver lysates (calculated MW of 28.6 kDa band according to NP_001017524.1). In transfected HEK293 transiently expressing SIRT3 bands of approx. 35 kDa band and 48 kDa band are observed. These bands are not observed in the non-transfected HEK293.
Positive Control
Liver Lysate (NB820-59232)
Sirtuin 3/SIRT3 Lysate (NBL1-15975)
Publications
Read Publications using
NB600-808 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Sirtuin 3/SIRT3 Antibody

  • EC 3.5.1
  • EC 3.5.1.-
  • hSIRT3
  • mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
  • NAD-dependent deacetylase sirtuin-3, mitochondrial
  • SIR2L3
  • SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
  • sir2-like 3
  • SIR2-like protein 3
  • SIRT3
  • sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
  • Sirtuin 3
  • sirtuin type 3

Background

This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1).

PMID: 7621916