Separase Antibody Summary
| Immunogen |
A synthetic peptide made to an internal region (within residues 1400-1500) of the human Separase protein. [Swiss-Prot# Q14674]
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| Localization |
Cytoplasmic and Nuclear.
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| Predicted Species |
Primate (93%). Backed by our 100% Guarantee.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
ESPL1
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| Purity |
Immunogen affinity purified
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| Innovators Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovators Reward
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This Separase antibody has only been used in preliminary studies including Peptide-ELISA.
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| Control Peptide |
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Reactivity Notes
Human. Immunogen sequence has 93% homology to monkey.
Packaging, Storage & Formulations
| Storage |
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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| Buffer |
TBS and 0.1% BSA
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| Preservative |
0.05% Sodium Azide
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| Concentration |
0.73 mg/ml
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| Purity |
Immunogen affinity purified
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Notes
Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at [email protected] for more details.
Alternate Names for Separase Antibody
- Caspase-like protein ESPL1
- EC 3.4.22.49
- ESP1extra spindle poles like 1
- extra spindle pole bodies homolog 1 (S. cerevisiae)
- extra spindle poles like 1 (S. cerevisiae)
- Extra spindle poles-like 1 protein
- FLJ46492
- KIAA0165separin
- Separase
Background
In normal cellular proliferation, the maintenance of constant centriole numbers is strongly associated with cell cycle process and the disengagement of mother-daugther centriole (a requirement for duplication of centriole) is facilitated by separase (ESPL1, a cysteine endopeptidase) mediated proteolytic cleavage of cohesin, a “glue” protein complex which is also essential to sister-chromatide cohesion. Separase is a caspase-like protease which plays a key role in chromosome segregation by cleaving SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle phases, separase is kept inactivated, and its proteolytic activity is tightly regulated by multiple inhibitory mechanisms combining securin binding, CyclinB1/Cdk1 mediated serine residue phosphorylation (pSer1226), PP2A binding and autocatalytic cleavage. Ectopic activation of separase proteolytic activity results in premature sister-chromatide separation/centriole disengagement and separase overexpression has been shown to induce aneuploidy and carcinogenesis.