Semaphorin 5A Antibody

Product: Tyrosine kinase inhibitor

Semaphorin 5A Antibody Summary

Immunogen
Peptide with sequence DEATKKACYSKGKS corresponding to internal region according to NP_003957.1.
Specificity
The immunizing peptide corresponds to a part of the extracellular domain.
Clonality
Polyclonal
Host
Goat
Gene
SEMA5A
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 – 6 ug/ml
  • Immunohistochemistry-Paraffin 4 – 6 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 90 kDa band in mouse brain lysates and approx. 25 kDa band in human brain (cerebellum), colon, heart and kidney lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 121 kDa band according to NP_003957.1. The 90 kDa band and 25 kDa band were successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows membanous and vesicular staining in PCT cells.
Publications
Read Publication using NB100-41107.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at [email protected] for more details.

Alternate Names for Semaphorin 5A Antibody

  • FLJ12815
  • sema domain, seven thrombospondin repeats (type 1 and type 1-like)
  • Sema F
  • SEMA5A
  • SEMAF
  • Semaphorin 5A
  • semaphorin F
  • semaphorin-5A
  • Semaphorin-F
  • semF
  • transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

Background

Members of the semaphorin protein family are involved in axonal guidance during neural development. Semaphorin 5a is thought to have a bifunctional role in axon guidance, exerting both attractive and inhibitory effects on developing axons associated with limbic function. The thrombospondin repeats of Semaphorin 5a physically interact with both heparan sulfate proteoglycans and chondroitin sulfate proteoglycans, inducing attractive and inhibitory guidance cues, respectively. The biological responses to Semaphorin 5a are induced through binding to its high-affinity receptor, plexin B3, triggering intracellular signaling through Met.

PMID: 17101327

Semaphorin 5A Antibody

Product: AG-490

Semaphorin 5A Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Semaphorin 5A. The exact sequence is proprietary.
Localization
Membrane; Single-pass type I membrane protein
Predicted Species
Mouse (97%), Rat (97%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEMA5A
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
121 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Semaphorin 5A Antibody

  • FLJ12815
  • sema domain, seven thrombospondin repeats (type 1 and type 1-like)
  • Sema F
  • SEMA5A
  • SEMAF
  • Semaphorin 5A
  • semaphorin F
  • semaphorin-5A
  • Semaphorin-F
  • semF
  • transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A

Background

Members of the semaphorin protein family, such as SEMA5A, are involved in axonal guidance during neural development (Adams et al., 1996 [PubMed 8817451]).[supplied by OMIM]

PMID: 17245358