SRGAP2 Antibody

Product: WIN 55,212-2 (Mesylate)

SRGAP2 Antibody Summary

Immunogen
Peptide with sequence C-KQEDRQTPRSPDST corresponding to internal region according to NP_056141.2.
Epitope
KQEDRQTPRSPDST – Internal region
Specificity
This antibody is expected to recognize isoform a (NP_056141.2).
Clonality
Polyclonal
Host
Goat
Gene
SRGAP2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 – 0.3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 – 1:2000
  • Peptide ELISA Detection limit 1:8000
Application Notes
IF: Transfected HEK293 transiently expressing GFP-tagged human srGAP2 with the primary antibody stained in the red channel (Confocal IF). WB: Approx. 124 kDa band observed in HeLa lysate and in rat primary culture cortical neurons at Day 4 (calculated MW of 121 kDa band according to human NP_056141.2, but the rat protein sequence is stlll provisional).
Publications
Read Publication using NBP1-49877.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at [email protected] for more details.

Alternate Names for SRGAP2 Antibody

  • FNBP2
  • Formin-binding protein 2
  • KIAA0456ARHGAP34formin binding protein 2
  • Rho GTPase-activating protein 34
  • SLIT-ROBO Rho GTPase activating protein 2
  • SLIT-ROBO Rho GTPase-activating protein 2
  • srGAP2
  • SRGAP3

Background

SRGAP2 is a putative GTPase-activating protein for Rho family small GTPases.

PMID: 15016804