SOD1/Cu-Zn SOD Antibody Summary
| Immunogen |
Human Cu/Zn SOD detects approx. 23kDa (human) and 19kDa (other species) proteins corresponding to the molecular mass of Cu/Zn superoxide dismutase (SOD) on SDS PAGE immunoblots
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| Localization |
Cytoplasm
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| Specificity |
Detects approx 23kDa (human) and approx 19kDa (other species).
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
SOD1
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
0.2 ug/ml of SOD (Cu/Zn) Antibody was sufficient for detection of Cu/Zn SOD in 20 ug of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary Antibody.
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| Positive Control |
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Reactivity Notes
Based on homology, It is predicted to detect Rat, Mouse, Bovine, Monkey, Invertebrate, Coral (Anthozoa), Canine, Hamster, Porcine, Rabbit, Sheep, Xenopus, Mollusk, Mussel (Perna viridis), Fish, Insect and Butterfly (Ops melastigma)
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS (pH 7.0) and 50% Glycerol
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| Preservative |
0.1% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for SOD1/Cu-Zn SOD Antibody
- ALS
- ALS1
- amyotrophic lateral sclerosis 1 (adult)
- Cu
- Cu/Zn superoxide dismutase
- CuZn SOD
- Cu-Zn SOD
- EC 1.15.1.1
- homodimer
- hSod1
- indophenoloxidase A
- Ipo1
- IPOA
- SOD
- SOD, cytosolic
- SOD, Soluble
- SOD1
- superoxide dismutase [Cu-Zn]
- Superoxide dismutase 1
- superoxide dismutase 1, soluble
- Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic
Background
Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2 to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(9). SOD3 can also be distinguished by its heparin-binding capacity (1).