SMC1 [p Ser957] Antibody Summary
| Immunogen |
The antiserum was produced against synthesized phosphopeptide derived from human SMC1 around the phosphorylation site of serine 957 (G-S-SP-Q-G).
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| Modification |
p Ser957
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| Specificity |
The antibody detects endogenous level of SMC1 only when phosphorylated at serine 957.
|
| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
SMC1A
|
| Purity |
Immunogen affinity purified
|
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Applications/Dilutions
| Dilutions |
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| Application Notes |
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
|
| Theoretical MW |
145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS pH 7.4 (without Mg2+ and Ca2+), 150mM NaCl and 50% glycerol.
|
| Preservative |
0.02% Sodium Azide
|
| Concentration |
1.0 mg/ml
|
| Purity |
Immunogen affinity purified
|
Alternate Names for SMC1 [p Ser957] Antibody
- CDLS2
- DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast)
- KIAA0178DKFZp686L19178
- SB1.8
- SB1.8MGC138332
- segregation of mitotic chromosomes 1
- SMC protein 1A
- SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
- SMC1 structural maintenance of chromosomes 1-like 1
- SMC1
- SMC1A
- SMC-1A
- SMC-1-alpha
- SMC1L1
- SMC1SMC1alpha
- Smcb
- structural maintenance of chromosomes 1A
- structural maintenance of chromosomes protein 1A
Background
Structural Maintenance of Chromosomes (SMC) family proteins play critical roles in various nuclear events that require structural changes of chromosomes, including mitotic chromosome organization, DNA recombination and repair and global transcriptional repression. The chromosome proteins are conserved in eukaryotes and can lead to mitotic chromosome segregation defects, suggesting a critical function of SMC family proteins in mitotic chromosome dynamics. SMC1 and SMC3 form a heterodimeric complex required for metaphase progression in mitotic cells. Specifically this SMC1/SMC3 complex is responsible for sister chromatid cohesion during metaphase. A number of cellular factors interact with hSMC1/hSMC3 during cell cycle. The major population of hSMC1/hSMC3 is in a compex with hRAD21 forming the human cohesion complex. Human cohesion complex associates with chromosomes which peaks at S phase and dissociates from chromosomes during G2/M transition. In addition, a subpopulation of hSMC1/hSMC3 associates tightly with nuclear matrix and centrosomes during interphase. A subset of hSMC1/hSMC3 is localized to spindle poles, spindles and kinetochores during mitosis when cohesin is in the cytoplasm. hSMC1/hSMC3 is required for spindle aster formation in vitro and reacts with nuclear mitotic apparatus protein in vivo.