SAP30L Antibody Summary
| Immunogen |
A synthetic peptide made to residues 165-183 (VKSNKSRLDQKSEGGKQLE) of human SAP30L.
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| Specificity |
This is specific for SAP30L.
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| Clonality |
Polyclonal
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| Host |
Rabbit
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| Gene |
SAP30L
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| Purity |
Immunogen affinity purified
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Applications/Dilutions
| Dilutions |
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| Application Notes |
This antibody has been tested in Western blot. No other applications have been tested. Recommended dilution below. Western Blot – 1:500 to 1:1,000* *However, the investigator should determine the optimal dilution. The transfected wild-type hSAP30L runs with an apparent molecular weight of ~30kDa and the endogenous hSAP30L band runs with an apparent molecular weight of 21-22 kDa. The difference in the size of the bands is unknown, but is seen by investigators in all studies of hSAP30L. Possible explanations for the size difference may be to cleavage product, degradation and/or post-translational modification.
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| Positive Control |
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| Publications |
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Reactivity Notes
This antibody reacts with wild-type transfected and endogenous human SAP30L protein. This antibody does not react with endogenous non-human SAP30L.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
PBS
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| Preservative |
0.02% Sodium Azide
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| Concentration |
1 mg/ml
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| Purity |
Immunogen affinity purified
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Alternate Names for SAP30L Antibody
- DKFZp667L2214
- FLJ11526
- FLJ36497
- HCV non-structural protein 4A-transactivated protein 2
- histone deacetylase complex subunit SAP30L
- NS4ATP2FLJ23595
- SAP30-like
- Sin3 corepressor complex subunit SAP30L
- Sin3A associated protein p30-like
- Sin3-associated protein p30-like
Background
A novel TGF- beta up-regulated mRNA species, the Sin3-associated protein 30-like, SAP30L has been identified. The predicted nuclear localization signal of SAP30L is sufficient for nuclear transport of the protein although mutating it does not completely remove SAP30L from the nuclei. By reason of its nuclear localization and close homology to SAP30 it is thought that SAP30L might have a role in recruiting the Sin3-histone deacetylase complex to specific co-repressor complexes in response to TGF-beta, leading to the silencing of proliferation-driving genes in the differentiating intestinal epithelial cells.