S100A9 Antibody (S100A9/1011) Summary
| Immunogen |
Recombinant human S100A9 protein
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| Localization |
Cytoplasmic, Extracellular (secreted) and Cell Surface
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| Marker |
Macrophage Marker
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| Specificity |
Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes and by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues).
|
| Isotype |
IgM Kappa
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
S100A9
|
| Purity |
Protein A or G purified
|
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|
Applications/Dilutions
| Dilutions |
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| Application Notes |
Hu-chromosome location: 1q21
Not suitable for Immunohistochemistry-Frozen tissues. Immunohistochemistry-Paraffin 0.5 – 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 – 20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Theoretical MW |
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C.
|
| Buffer |
10mM PBS
|
| Preservative |
0.05% Sodium Azide
|
| Concentration |
0.2 mg/ml
|
| Purity |
Protein A or G purified
|
Alternate Names for S100A9 Antibody (S100A9/1011)
- CAGBMigration inhibitory factor-related protein 14
- Calgranulin B
- calgranulin-B
- Calprotectin L1H subunit
- CFAGMRP-14,60B8AG
- CGLB
- L1AG
- LIAG
- MAC387
- MIF
- MRP-14
- MRP14Leukocyte L1 complex heavy chain
- NIF
- P14
- protein S100-A9
- S100 calcium binding protein A9 (calgranulin B)
- S100 calcium binding protein A9
- S100 calcium-binding protein A9 (calgranulin B)
- S100 calcium-binding protein A9
- S100A9