Rhodopsin Antibody (1D4) Summary
| Immunogen |
Bovine Rhodopsin
|
| Localization |
Membrane
|
| Specificity |
Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
|
| Isotype |
IgG1
|
| Clonality |
Monoclonal
|
| Host |
Mouse
|
| Gene |
RHO
|
| Purity |
Protein G purified
|
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|
Applications/Dilutions
| Dilutions |
|
| Application Notes |
1 ug/ml of this antibody was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
|
| Theoretical MW |
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
|
| Buffer |
PBS pH7.4, 50% glycerol
|
| Preservative |
0.09% Sodium Azide
|
| Concentration |
1 mg/ml
|
| Purity |
Protein G purified
|
Alternate Names for Rhodopsin Antibody (1D4)
- CSNBAD1
- MGC138309
- OPN2MGC138311
- opsin 2, rod pigment
- opsin-2
- retinitis pigmentosa 4, autosomal dominant
- rhodopsin
- RP4
Background
Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsins activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).