Rev-erb beta/NR1D2 Antibody

Product: GSK2330672

Rev-erb beta/NR1D2 Antibody Summary

Immunogen
Synthetic 16 amino acid peptide from internal region of human NR1D2.
Specificity
Human NR1D2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Species
Turkey (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
NR1D2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:100 – 1:2000
  • Immunohistochemistry-Paraffin 10 ug/ml
Application Notes
IHC: NBP1-50165 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after proteinase K antigen retrieval. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Reactivity Notes

Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Panda (100%), Elephant (94%), Platypus (94%), Opossum (81%), Xenopus (81%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rev-erb beta/NR1D2 Antibody

  • BD73
  • EAR-1R
  • HZF2
  • NR1D2
  • nuclear receptor subfamily 1 group D member 2
  • nuclear receptor subfamily 1, group D, member 2
  • Orphan nuclear hormone receptor BD73
  • Reverb beta
  • Rev-erb beta
  • rev-erba-alpha-related receptor
  • rev-erb-beta
  • RVR
  • V-erbA-related protein 1-related

PMID: 11959792

Rev-erb beta/NR1D2 Antibody

Product: EN460

Rev-erb beta/NR1D2 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: SAMKTMMNSQFSGHLQNDTLVEHHEQTALPAQEQLRPKPQLEQENIKSSSPPSSDFAKEEVIGMVTRAHKDTFMYNQEQQENSAESMQPQRGERIPKN
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NR1D2
Purity
Immunogen affinity purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

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Applications/Dilutions

Dilutions
  • Western Blot 1:250 – 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500 – 1:1000
Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended.
Control Peptide
Rev-erb beta/NR1D2 Protein (NBP2-47306PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (82%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Rev-erb beta/NR1D2 Antibody

  • BD73
  • EAR-1R
  • HZF2
  • NR1D2
  • nuclear receptor subfamily 1 group D member 2
  • nuclear receptor subfamily 1, group D, member 2
  • Orphan nuclear hormone receptor BD73
  • Reverb beta
  • Rev-erb beta
  • rev-erba-alpha-related receptor
  • rev-erb-beta
  • RVR
  • V-erbA-related protein 1-related

PMID: 22644306