Product: Ivabradine metabolite N-Demethyl Ivabradine (hydrochloride)
RelA/NFkB p65 Antibody (NF-12) Summary
| Immunogen |
Recombinant, mouse NF-kB p65 fragment (C-terminal 151 amino acids).
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| Localization |
Cytoplasmic and Nuclear
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| Specificity |
This recognizes an epitope within the C-terminal region of the mouse NF-kappaB p65.
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| Isotype |
IgG1
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| Clonality |
Monoclonal
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| Host |
Mouse
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| Gene |
RELA
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| Purity |
Unpurified
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| Innovators Reward |
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Applications/Dilutions
| Dilutions |
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| Application Notes |
In WB, detects a band of approximately 65 kDa. A minimum working dilution of 1:1000 is determined by western blotting using a whole cell extract of 3T3 mouse fibroblast cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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| Theoretical MW |
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Positive Control |
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| Publications |
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Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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| Buffer |
Ascites
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| Preservative |
15mM Sodium Azide
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| Purity |
Unpurified
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Alternate Names for RelA/NFkB p65 Antibody (NF-12)
- MGC131774
- nf kb p65
- NFkB p65
- NF-kB p65
- NFKB3
- NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65))
- Nuclear factor NF-kappa-B p65 subunit
- Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65
- p65
- p65RelA
- rela p65
- RelA
- v-rel reticuloendotheliosis viral oncogene homolog A (avian)
- v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight polypeptide gene enhancer in B-cells 3, p65
Background
p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear accumulation of NFkB by the induced phosphorylation and subsequent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimers disease, atherosclerosis and cancer.