Product: 6-Quinoxalinecarboxylic acid, 2,4-bis(bromomethyl)-
Recombinant Human Thioredoxin Protein Summary
Description |
An un-tagged recombinant protein corresponding to amino acids 1 to 105 of Human Thioredoxin
Source: E.coli Amino Acid Sequence: MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV |
Preparation Method |
E.coli
|
Details of Functionality |
Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
|
Protein/Peptide Type |
Recombinant Protein
|
Gene |
TXN
|
Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
Application Notes |
Assay
1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately. 3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes |
Theoretical MW |
11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
Buffer |
Phosphate buffer saline (pH 7.4)
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Preservative |
No Preservative
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Concentration |
1.0 mg/ml
|
Purity |
>90%, by SDS-PAGE
|
Notes
Alternate Names for Recombinant Human Thioredoxin Protein
- ADF
- ATL-derived factor
- MGC61975
- SASP
- Surface-associated sulphydryl protein
- thioredoxin
- TRDX
- Trx
- TRX1
- TRXDKFZp686B1993