Recombinant Human Thioredoxin Protein

Product: 6-Quinoxalinecarboxylic acid, 2,4-bis(bromomethyl)-

Recombinant Human Thioredoxin Protein Summary

Description
An un-tagged recombinant protein corresponding to amino acids 1 to 105 of Human Thioredoxin

Source: E.coli

Amino Acid Sequence: MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV

Preparation
Method
E.coli
Details of Functionality
Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
Protein/Peptide Type
Recombinant Protein
Gene
TXN
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Application Notes
Assay
1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA.
2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately.
3. Incubate at 25C for 20 minutes.
4. Record the increase in A650nm for 15 minutes
Theoretical MW
11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Phosphate buffer saline (pH 7.4)
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
>90%, by SDS-PAGE

Notes

The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 11.7 kDa (105 aa), confirmed by MALDI-TOF.

Alternate Names for Recombinant Human Thioredoxin Protein

  • ADF
  • ATL-derived factor
  • MGC61975
  • SASP
  • Surface-associated sulphydryl protein
  • thioredoxin
  • TRDX
  • Trx
  • TRX1
  • TRXDKFZp686B1993

Background

Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques

PMID: 18451518