Product: 6-Quinoxalinecarboxylic acid, 2,4-bis(bromomethyl)-
Recombinant Human Thioredoxin Protein Summary
| Description |
An un-tagged recombinant protein corresponding to amino acids 1 to 105 of Human Thioredoxin
Source: E.coli Amino Acid Sequence: MVKQIESKTA FQEALDAAGD KLVVVDFSAT WCGPCKMIKP FFHSLSEKYS NVIFLEVDVD DCQDVASECE VKCMPTFQFF KKGQKVGEFS GANKEKLEAT INELV |
| Preparation Method |
E.coli
|
| Details of Functionality |
Specific activity is >150 A650/cm/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
TXN
|
| Purity |
>90%, by SDS-PAGE
|
Applications/Dilutions
| Application Notes |
Assay
1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin into reaction cocktail and mix immediately. 3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes |
| Theoretical MW |
11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
Phosphate buffer saline (pH 7.4)
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Notes
The purity of this protein is > 95% by SDS-PAGE. Molecular weight is 11.7 kDa (105 aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human Thioredoxin Protein
- ADF
- ATL-derived factor
- MGC61975
- SASP
- Surface-associated sulphydryl protein
- thioredoxin
- TRDX
- Trx
- TRX1
- TRXDKFZp686B1993
Background
Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. It is involved in the first unique step in DNA synthesis. Trx also provides control over a number of transcription factors affecting cell proliferation and death through a mechanism referred to as redox regulation. Recombinant human thioredoxin-1 was overexpressed in E. coli and purified by using conventional chromatography techniques