Recombinant Human HMGB1/HMG-1 Protein

Product: NH3-QGGYTMHQDQEGDTDAGLK-COOH

Recombinant Human HMGB1/HMG-1 Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 215 of Human HMGB1/HMG-1

Source: Baculovirus

Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH

Preparation
Method
Hi-5
Protein/Peptide Type
Recombinant Protein
Gene
HMGB1
Purity
>90%, by SDS-PAGE
Endotoxin Note
< 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
20mM Tris pH 8.0, 1mM EDTA, 0.5mM DTT, 10% glycerol
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
>90%, by SDS-PAGE

Notes

The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 25kDa (223aa), confirmed by MALDI-TOF.

Alternate Names for Recombinant Human HMGB1/HMG-1 Protein

  • Amphoterin
  • high mobility group box 1
  • High mobility group protein 1
  • high mobility group protein B1
  • high-mobility group (nonhistone chromosomal) protein 1
  • high-mobility group box 1
  • HMG1
  • HMG-1
  • HMG1DKFZp686A04236
  • HMG3
  • HMGB1
  • SBP-1
  • Sulfoglucuronyl carbohydrate binding protein

Background

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in High Five insect cells and purified by conventional chromatography techniques.

PMID: 21923175