Product: NH3-QGGYTMHQDQEGDTDAGLK-COOH
Recombinant Human HMGB1/HMG-1 Protein Summary
| Description |
A recombinant protein with a His-tag corresponding to amino acids 1 to 215 of Human HMGB1/HMG-1
Source: Baculovirus Amino Acid Sequence: MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEED EEDEEDEEEE EDEEDEDEEE DDDDELEHHH HHH |
| Preparation Method |
Hi-5
|
| Protein/Peptide Type |
Recombinant Protein
|
| Gene |
HMGB1
|
| Purity |
>90%, by SDS-PAGE
|
| Endotoxin Note |
< 1.0 EU per 1 microgram of protein (determined by LAL method)
|
Applications/Dilutions
| Theoretical MW |
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles.
|
| Buffer |
20mM Tris pH 8.0, 1mM EDTA, 0.5mM DTT, 10% glycerol
|
| Preservative |
No Preservative
|
| Concentration |
1.0 mg/ml
|
| Purity |
>90%, by SDS-PAGE
|
Notes
The purity of this protein is > 90% by SDS-PAGE. Molecular weight is 25kDa (223aa), confirmed by MALDI-TOF.
Alternate Names for Recombinant Human HMGB1/HMG-1 Protein
- Amphoterin
- high mobility group box 1
- High mobility group protein 1
- high mobility group protein B1
- high-mobility group (nonhistone chromosomal) protein 1
- high-mobility group box 1
- HMG1
- HMG-1
- HMG1DKFZp686A04236
- HMG3
- HMGB1
- SBP-1
- Sulfoglucuronyl carbohydrate binding protein
Background
High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in High Five insect cells and purified by conventional chromatography techniques.