Recombinant Human Glucokinase/GCK Protein

Product: Gilteritinib

Recombinant Human Glucokinase/GCK Protein Summary

Description
A recombinant protein corresponding to 470 amino acids of Human pancreas Glucokinase/GCK

Source: E. coli.

Amino Acid Sequence: The amino acid sequence of this product is not available.

Preparation
Method
E. coli
Details of Functionality
303 pmol/min/ug. One unit is defined as the amount of enzyme that will convert 1 pmol of NADP to NADPH at 30oC. Assay conditions: 25 mM HEPES, pH 7.5, 2 mM MgCl2, 1.0 mM DTT, 0.5 mM NADP, 2.0 mM ATP, 25 mM glucose, 100ug/ml BSA, 20 units/ml glucose 6-phosphate dehydrogenase, and 10 nM human pancreatic glucokinase at 30C for 30 min.
Protein/Peptide Type
Recombinant Protein
Gene
GCK

Applications/Dilutions

Theoretical MW
53.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
50 mM potassium phosphate pH 7.4, 50 mM sodium chloride, 0.5 mM DTT, 0.5 mM EDTA, and 2.5% glycerol.
Concentration
1 mg/ml

Alternate Names for Recombinant Human Glucokinase/GCK Protein

  • EC 2.7.1
  • EC 2.7.1.2
  • FGQTL3
  • GCK
  • GK
  • GLK
  • glucokinase (hexokinase 4)
  • Glucokinase
  • Hexokinase-4
  • Hexokinase-D
  • HHF3
  • HK4
  • HK4pancreatic isozyme
  • HKIV
  • HXKP
  • LGLK
  • maturity onset diabetes of the young 2
  • MODY2

Background

Glucose is phosphorylated to glucose-6-phosphate by glucokinases. This gene is alternatively spliced to generate three different forms of the enzyme; one found in the pancreas and two found in the liver. The main function of this gene is to regulate carbohydrate metabolism. Recombinant human pancreatic Glucokinase (Accession# NP_000153)) has a C-terminal FLAG tag and has 470 amino acid residues. It can be useful for studies including enzyme kinetics, activator screening and kinase selectivity.

PMID: 12351713